Clinical Journal of Obstetrics and Gynecology Research

Expression of CK-7, CA125 and Survivin in Paraneoplastic Tissues at Different Distances from Orthotopic Human Epithelial Ovarian Carcinomas in Nude Mice

Gen-Hai Zhu*, Xin-Hui Fu, Fu-Jin Liu, Chun-Ying Chen, Hai-Yan Huang, Lan Hong, Kang Wang, Sheng-Tan Wang and Lang Zheng

Abstract: 

Objective: The objective of this study was to investigate the safety margin of ovary-sparing local tumorectomy by detecting the expression of CK-7, CA125 and survivin in paraneoplastic tissues at different distances from orthotopic human epithelial ovarian carcinomas in nude mice. Methods: RT-PCR and IHC were used to measure the expression levels of CK-7, CA125 and survivin in the ovarian tissues of 20 normal nude mice as well as in the cancer tissue and paraneoplastic tissues (respectively 1 mm - 1.5 mm, 1.5 mm – 3 mm, 3 mm - 4.5 mm from the cancer) of 18 nude mice with orthotopic human epithelial ovarian carcinomas. Results: According to the RT-PCR and IHC results, the expression levels or positive rates of CK-7, CA125 and survivin in the ovarian tissue 1 mm - 1.5 mm from the cancer were significantly higher than those in the normal tissue (P<0.01), but slightly lower than those in the cancer tissue with no statistical difference (P>0.05); the expression levels or positive rates in the ovarian tissue 1.5 mm – 3 mm from the cancer were significantly lower than those in the cancer tissue (P<0.01), but slightly higher than those in the normal tissue with no statistical difference (P>0.05); the expression levels or positive rates in the ovarian tissue 3 mm - 4.5 mm from the cancer were significantly higher than those in the normal tissue (P<0.01), but slightly lower than those in the cancer tissue with no statistical difference (P>0.05); there were no statistically significant differences in the expression levels or positive rates between the ovarian tissue 3 mm - 4.5 mm from the cancer and the normal ovarian tissue (P>0.05). Conclusions: The expression levels or positive rates of CK-7, CA125 and survivin in the paraneoplastic tissues showed a decreasing trend towards the non-cancer side; the tissue 1 mm - 1.5 mm from the cancer may be an area with a risk of occult metastasis and 3 mm is the minimum safe distance for performing ovary-sparing local tumorectomy for orthotopic human epithelial ovarian carcinomas in nude mice. 

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