Journal of Nanotechnology Open Access

Assembly and Study of Mesenchymal Stem Cells on Controlled Chemistry Surfaces

Batoul Hamieh, Cristina Vochitoaia, Halima Alem, Emmanuel Guedon, Tayssir Hamieh and Joumana Toufaily 

Abstract:

The cell responds to the physical constraints exerted by its environment by a set of mechanisms grouped under the term of mechanotransduction. These processes involve the molecules involved in cell adhesion, the cytoskeleton and the nucleus. These environmental constraints, whether related to the rigidity of the support, to its topography or to the nature of its surface chemistry, will modulate the cellular morphology and impact the behavior of the cell. In order to study this influence of the support, we have seeded bone marrow mesenchymal stem cells from a primary culture of a 24 years old individual on virgin mica surfaces or treated homogeneously with natural molecules (fibronectin and the cyclic RGD peptide) or with polyelectrolyte multilayers (five cycles of Chitosan / PAA or Chitosan / PSS). We then studied the morphology, proliferation and differentiation of these cells after 12 days of culture. As a result, bone marrow mesenchymal stem cells adhere to all surfaces, whether treated or not, and although they are less spread on virgin surfaces, they adopt a fibroblastic type morphology similar to their physiological phenotype. Their percentage of confluence varies significantly depending on the surface treatment used. Indeed the maximum confluence was observed for the surfaces grafted with fibronectin (93.25 ± 2.75%) whereas the surfaces treated with the polyelectrolyte multilayers have much lower confluence percentages (61.00 ± 4.08% for the chitosan / PAA couple) and 54.75 ± 1.75% for the Chitosan / PSS couple), mainly due to cell latency at the beginning of culture. Finally, cells cultured on our surfaces do not respond to any of the three Oil Red O, Alcian Blue or Alizarin Red S stains, suggesting a lack of differentiation in the adipogenic, chondrogenic or osteogenic pathways induced by these surfaces. Thus, the control of the support chemistry alone does not allow control of cell differentiation.

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Clinical Journal of Obstetrics and Gynecology Research

Expression of CK-7, CA125 and Survivin in Paraneoplastic Tissues at Different Distances from Orthotopic Human Epithelial Ovarian Carcinomas in Nude Mice

Gen-Hai Zhu*, Xin-Hui Fu, Fu-Jin Liu, Chun-Ying Chen, Hai-Yan Huang, Lan Hong, Kang Wang, Sheng-Tan Wang and Lang Zheng

Abstract: 

Objective: The objective of this study was to investigate the safety margin of ovary-sparing local tumorectomy by detecting the expression of CK-7, CA125 and survivin in paraneoplastic tissues at different distances from orthotopic human epithelial ovarian carcinomas in nude mice. Methods: RT-PCR and IHC were used to measure the expression levels of CK-7, CA125 and survivin in the ovarian tissues of 20 normal nude mice as well as in the cancer tissue and paraneoplastic tissues (respectively 1 mm - 1.5 mm, 1.5 mm – 3 mm, 3 mm - 4.5 mm from the cancer) of 18 nude mice with orthotopic human epithelial ovarian carcinomas. Results: According to the RT-PCR and IHC results, the expression levels or positive rates of CK-7, CA125 and survivin in the ovarian tissue 1 mm - 1.5 mm from the cancer were significantly higher than those in the normal tissue (P<0.01), but slightly lower than those in the cancer tissue with no statistical difference (P>0.05); the expression levels or positive rates in the ovarian tissue 1.5 mm – 3 mm from the cancer were significantly lower than those in the cancer tissue (P<0.01), but slightly higher than those in the normal tissue with no statistical difference (P>0.05); the expression levels or positive rates in the ovarian tissue 3 mm - 4.5 mm from the cancer were significantly higher than those in the normal tissue (P<0.01), but slightly lower than those in the cancer tissue with no statistical difference (P>0.05); there were no statistically significant differences in the expression levels or positive rates between the ovarian tissue 3 mm - 4.5 mm from the cancer and the normal ovarian tissue (P>0.05). Conclusions: The expression levels or positive rates of CK-7, CA125 and survivin in the paraneoplastic tissues showed a decreasing trend towards the non-cancer side; the tissue 1 mm - 1.5 mm from the cancer may be an area with a risk of occult metastasis and 3 mm is the minimum safe distance for performing ovary-sparing local tumorectomy for orthotopic human epithelial ovarian carcinomas in nude mice. 

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