Linezolid Resistant Coagulase Negative Staphylococci Isolated from Clinical Specimens in A Tertiary Care Cardiac Hospital, Pakistan
Arif Maqsood Ali*1, Gul e Raana Waseem2, Shazia Arif 3
Abstract
Background: The
objective of this study was to determine the emergence of Linezolid resistance
among Coagulase Negative Staphylococcus in patients of tertiary healthcare
facility at Rawalpindi Institute of Cardiology (RIC), Pakistan.
Method: A retrospective study was carried
out to find Linezolid Resistance Coagulase Negative Staphylococcus (LZRC) from
clinical specimens in microbiology department of Rawalpindi institute of
cardiology from year January 2016 to October 2018. The clinical specimens
included blood, wound swab, pus swab, bronchial secretions, urine, catheter
tip, suction tip, central venous (CVP) tip, Temporary Pace Maker (TPM) tip and
valvular vegetation. These specimens were collected from indoor & outdoor
patients and cultured in microbiology laboratory. Culture media used to isolate
Coagulase Negative Staphylococci (CoNS) included Blood Agar and MacConkey Agar.
The organisms were identified by standard microbiological methods. Antibiotic
susceptibility was performed by modified disc diffusion & Vitek 2 methods
and confirmed on Microbroth Dilution (MBD) according to CLSI Guidelines.
Results: A total of 104 specimens yielded
CoNS. Four of them were LZRC (3.84%) isolated in blood, pus, bronchial
secretions and urine specimens. Three were isolated in specimens from male
patients and one sample from a female patient. Out of these four positive LZRC
isolates two were Staphylococcus haemolyticus.
Conclusions: The findings of LZRC
isolated at RIC is comparatively higher than other studies worldwide. Judicious
use of this antibiotic along with infection control measures is crucial to
control antibiotic resistance so that the availability of linezolid in life
threatening infections is not compromised.
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